LRC Chromatography Columns for Laboratory Applications

Empty glass columns accommodate sorbent volumes up to 900 mL for preparative and process optimization applications

Robust Design

• Tubing connections for the inlet and outlet are on the column exterior to provide instant visual confirmation of integrity, and permit quick change of tubing and fittings.
• The frits provide even flow distribution across the bed with no hold-up volume behind the frits.
• High pressure resistance across the range of column diameters.

Precise Piston Adjustment

• Screw-lock system allows rapid coarse adjustment followed by precise fine adjustment of the piston.
• Piston motion is completely linear and provides true axial compression without torsional damage to the top of the column bed.
• Piston adjustment with the screw-lock system does not twist tubing or cause damage to the tubing connections.

Ease of Use

• Use of one or two adjustable pistons gives coverage of the complete range of bed heights from 0 to 750 mm.
• 1/4-28 UNF fittings are compatible with or adaptable to any liquid chromatography system.
• Column end couplings permit easy disassembly of end fittings for unpacking and cleaning operations.
• Frit replacement is quick and easy.
• Reservoir available for packing operations where slurry volume exceeds the column volume.

Broad Capabilities

• Extended pressure capabilities. (See Performance tab.)
• Temperature range: 4 – 40 °C.

Four internal diameters from 10 to 50 mm and four lengths provide a range of column volumes up to 900 mL and bed heights up to 750 mm. Column tubes are made of borosilicate glass. They are equipped with one adjustable and one fixed piston, and a screw-lock system to allow rapid adjustment of the piston. To allow a broader range of packed bed heights and volumes, the fixed piston may be replaced by a second adjustable piston. Spare adjustable pistons are available for each column diameter from 10 to 50 mm. Reservoirs (consisting of a column coupler and a glass tube) are available for packing.

Each column is supplied assembled and ready for use, with tubing and fittings to connect the column to a chromatography system.

Column Components and Materials of Construction

Description	Diagram Reference Number (See diagram below)	Materials of Construction
Column Tube	1	Borosilicate glass
Pistons: 1 adjustable and 1 fixed	2 3
Each with:
Internal central screw	4	POM(a)
Seal actuation nut	5	POM
Central screw	6	POM
Counter nuts and counter screw	7 10	POM POM
Washer	8	POM
Spring washer	9	Steel
O-ring seal	11	FPM(b)
10 µm frit (pressed into column piston)	12	Sintered glass
Standard union (1/4-28 UNF both sides)(c)	13	Polypropylene

Detail of A Pall LRC Column

Parts Included in Column Package

Quantity	Description	Materials of Construction
2	Fitting (ferrule with nut)	POM and Tefzel*
1	Connection tubing (1/16 in. or 1/8 in.OD)	FEP(d)
1	Frit ejector	POM and steel
1	User Guide No. USD 2482	-

(a) POM: Polyoxymethylene (acetal)
(b) Fluoroelastomer polymer

(c) Only on columns of I.D. 10 and 15 mm
(d) FEP: Fluorinated ethylene propylene

LRC empty glass columns allow researchers and process developers to quickly and easily evaluate different sorbents early in the drug development process to obtain necessary scale-up data.

LRC Column	ID (mm)	Equipped with 1 Adjustable Piston		Equipped with 2 Adjustable Pistons		Maximum Pressure Tested
		Bed Height (mm) min – max	Volume (mL) min – max	Bed Height (mm) min – max	Volume (mL) min – max	(barg)	(psig)
LRC Column 10/0 – 120	10	0 – 120	0 – 9	0 – 120	0 – 9	30	435
LRC Column 10/80 – 200	10	80 – 200	6 – 16	0 – 200	0 – 16	30	435
LRC Column 10/330 – 450	10	330 – 450	26 – 35	210 – 450	17 – 35	30	435
LRC Column 15/80 – 200	15	80 – 200	14 – 35	0 – 200	0 – 35	25	360
LRC Column 15/330 – 450	15	330 – 450	58 – 80	210 – 450	37 – 80	25	360
LRC Column 15/630 – 750	15	630 – 750	111 – 133	111 – 133	90 – 133	25	360
LRC Column 25/80 – 200	25	80 – 200	39 – 98	0 – 200	0 – 98	15	215
LRC Column 25/330 – 450	25	330 – 450	162 – 221	210 – 450	103 – 221	15	215
LRC Column 25/630 – 750	25	630 – 750	309 – 368	510 – 750	250 – 368	15	215
LRC Column 50/80 – 200	50	80 – 200	157 – 393	0 – 200	0 – 393	10	145
LRC Column 50/330 – 450	50	330 – 450	648 – 884	210 – 450	412 – 884	10	145

Pall offers a comprehensive line of chromatography columns from lab to process scale.

	PRC Columns 1 - 5 mL (prepacked) Fast selectivity screening with Q and S HyperCel Ion Exchange Sorbents with Q and CM Ceramic HyperD Ion Exchange Sorbents with MEP, HEA, and PPA HyperCel Mixed-mode Sorbents
	LRC Columns 5 - 900 mL Preparative and process optimization
	Resolute® Columns 6 - 300 L Pilot and small-scale production
	Resolute Columns 50 - 1500 L Large-scale production

Columns

Part Number	Description
LRC10x000-120V01	LRC Column 10/0 – 120
LRC10x080-200V01	LRC Column 10/80 – 200
LRC10x330-450V01	LRC Column 10/330 – 450
LRC15x080-200V01	LRC Column 15/80 – 200
LRC15x330-450V01	LRC Column 15/330 – 450
LRC15x630-750V01	LRC Column 15/630 – 750
LRC25x080-200V01	LRC Column 25/80 – 200
LRC25x330-450V01	LRC Column 25/330 – 450
LRC25x630-750V01	LRC Column 25/630 – 750
LRC50x080-200V01	LRC Column 50/80 – 200
LRC50x330-450V01	LRC Column 50/330 – 450

Spare Part Kits(1)

Part Number	Description
LRC10 KITV01	LRC Spare Part Lit for Column ID10
LRC15 KITV01	LRC Spare Part Kit for Column ID15
LRC25 KITV01	LRC Spare Part Kit for Column ID25
LRC50 KITV01	LRC Spare Part Kit for Column ID50

Glass Tubes

Part Number	Description
LRC10XTUBE000-120	LRC Glass Tube 10/0 – 120
LRC10XTUBE080-200	LRC Glass Tube 10/80 – 200
LRC10XTUBE330-450	LRC Glass Tube 10/330 – 450
LRC15XTUBE080-200	LRC Glass Tube 15/80 – 200
LRC15XTUBE330-450	LRC Glass Tube 15/330 – 450
LRC15XTUBE630-750	LRC Glass Tube 15/630 – 750
LRC25XTUBE080-200	LRC Glass Tube 25/80 – 200
LRC25XTUBE330-450	LRC Glass Tube 25/330 – 450
LRC25XTUBE630-750	LRC Glass Tube 25/630 – 750
LRC50XTUBE080-200	LRC Glass Tube 50/80 – 200
LRC50XTUBE330-450	LRC Glass Tube 50/330 – 450

Column Couplers (2)

Part Number	Description
LRC10XCOUPLERV01	Column Coupler for LRC Column ID10
LRC15XCOUPLERV01	Column Coupler for LRC Column ID15
LRC25XCOUPLERV01	Column Coupler for LRC Column ID25
LRC50XCOUPLERV01	Column Coupler for LRC Column ID50

Adjustable Pistons

Part Number	Description
LRC10ADJPLUNGERV01	LRC Adjustable Piston ID10
LRC15ADJPLUNGERV01	LRC Adjustable Piston ID15
LRC25ADJPLUNGERV01	LRC Adjustable Piston ID25
LRC50ADJPLUNGERV01	LRC Adjustable Piston ID50

1) One Spare kit includes: 4 O-rings, 4 frits of 10 µm, 1 m tubing.
(2) For a reservoir: order a glass tube and a column coupler.