Nucleic Acid and Protein Detection Products

Nylon membranes offer superior performance with both radioactive and nonradioactive detection systems. Pall's Biodyne nylon membranes will not crack, shrink or tear when subjected to multiple cycles of hybridization, stripping and reprobing. These membranes are instrinsically hydrophilic for easy wetting.

Product	Biodyne® A Membrane	Biodyne B Membrane Biodyne Plus Membrane	Biodyne C Membrane
Description	Amphoteric Nylon 6,6	Positively-charged Nylon 6,6	Negatively-charged Nylon 6,6
Works best for:	Colony/Plaque Lifts, DNA and RNA Transfers	DNA and RNA Transfers, Multiple Reprobings	Reverse Dot Blots
Also suited for:	Gene Probe Assays, DNA Fingerprinting, Nucleic Acid Dot/Slot Blots, Replica Plating, ELISAs	DNA Fingerprinting, Nucleic Acid Dot/Slot Blots, Colony/Plaque Lifts (Biodyne B membrane), Replica Plating (Biodyne B membrane)	Protein Immobilization, Affinity Purification, ELISAs
Advantages	High sensitivity, Low background, Net charge can be controlled by changing pH	Positive charge over broad pH range, Highest sensitivity for nucleic acid applications (Biodyne B membrane), No need to bake or UV crosslink	Negative charge over broad pH range, Surface carboxyl groups can be derivatized
Binding Interaction	Hydrophobic and Electrostatic	Electrostatic	Electrostatic
Method of Immobilization	UV Crosslink, Baking	UV Crosslink, Baking (neither required)	Derivatization
Detection Methods	Radiolabeled Probes, Enzyme-antibody, Conjugates, Chemiluminescent, Chromogenic	Radiolabeled Probes, Enzyme-antibody Conjugates, Chemiluminescent, Chromogenic	Radiolabeled Probes, Enzyme-antibody Conjugates, Chromogenic

FluoroTrans®, FluoroTrans W, and BioTrace™ PVDF membranes are hydrophobic in nature and strongly bind proteins of interest. FluoroTrans membrane is recommended for protein sequencing and is compatible with all reagents involved in the Edman reaction. FluoroTrans W and BioTrace PVDF membranes exhibit low background and high tensile strength, and are the best membranes available for detection of protein after Western transfer. All FluoroTrans membranes exhibit exceptionally low burn through.

Product	BioTrace PVDF Membrane	FluoroTrans® Membrane	AcroWell™ Filter Plates with BioTrace PVDF Membrane
Description	Polyvinylidene Fluoride	Polyvinylidene Fluoride	Polyvinylidene Fluoride
Works best for:	Protein Transfers	FluoroTrans W: Western Transfers, FluoroTrans: N-terminal Protein Sequencing	Protein Detection, ELISAs
Also suited for:	Nucleic Acid Transfers, Protein Dot/Slot Blots	Southern Transfers	Dot Blots, Protein:Protein Interactions
Advantages	Chemical resistance, No discoloration, Nonflammable, High strength	Strong protein binding, Sensitive detection, Very low burn-through, Good chemical compatibility	High binding capacity, Chemical resistance, 96 well SBS-format, Choice of housing colors
Binding Interaction	Hydrophobic	Hydrophobic	Hydrophobic
Method of Immobilization	UV Crosslink, Baking, Alkaline Fixation	UV Crosslink, Baking, Alkaline Fixation	Incubation and Vacuum Filtration
Detection Methods	Direct Stain, Enzyme-antibody Conjugates, Chemiluminescent, Chromogenic	Direct Stain (FluoroTrans W), Enzyme-antibody Conjugates, Chemiluminescent, Chromogenic, Fluorescent (FluoroTrans PVDF)	Chemiluminescent, Chromogenic, Fluorescent

BioTrace NT pure nitrocellulose membrane can be used for Western transfers and ELISpot assays. The membrane offers high protein binding and is compatible with a wide range of protein stains and immunodetection techniques.

Product	BioTrace NT Membrane	AcroWell™ Filter Plates with BioTrace NT Membrane
Description	100% Pure Nitrocellulose	100% Pure Nitrocellulose
Works best for:	Colony/Plaque Lifts	Dot Blots, ELISAs
Also suited for:	Nucleic Acid and Protein Transfers, Protein Dot/Slot Blots	Protein Dot Blots
Advantages	Excellent strength, No support fabric, No detergents added, 100% pure nitrocellulose	High binding capacity, 96 well SBS-format, Choice of housing colors
Binding Interaction	Hydrophobic and Electrostatic	Hydrophobic and Electrostatic
Method of Immobilization	UV Crosslink, Baking	Incubation and Vacuum Filtration
Detection Methods	Radiolabeled Probes, Direct Stain, Fluorescence, Enzyme-antibody Conjugates, Chemiluminescent, Chromogenic	Chemiluminescent, Chromogenic, Radiolabeled Probes, Fluorescent

Pall offers two activated membranes for covalent protein binding: UltraBind™ modified polyethersulfone has a high ratio of covalent to non-covalent protein binding; Immunodyne® ABC membrane offers the sharp spot geometry of nylon with a proprietary covalent attachment chemistry.

Product	UltraBind™ Membrane	Immunodyne™ ABC Membrane
Description	Modified Polyethersulfone	Modified Nylon 6,6
Works best for:	Solid-phase ELISAs	Immuno Assays, Diagnostic Applications, Reverse Dot Blots
Also suited for:	Affinity Chromatography, Hybridoma Screening	Enzyme Biosensor Applications, ELISAs
Advantages	Permanent binding, No preactivation required, High protein-binding capacity	Activated surface, No preactivation required, Intrinsically hydrophilic
Binding Interaction	Covalent to Amine Groups	Covalent to Amine and Carboxyl Groups
Method of Immobilization	Air Drying	Air Drying
Detection Methods	Radiolabeled Probes, Enzyme-antibody Conjugates, Chromogenic	Chemiluminescent, Chromogenic, Radiolabeled Probes, Fluorescent

• Using Membranes to Obtain High Sensitivities in Nucleic Acid and Protein Detection
• Transfer and Detection Procedures for Pall Life Sciences Membranes
• A Model for Binding of DNA and Proteins to Transfer Membranes
• Protein Sample Preparation and Analysis Application Manual - Analysis
• Northern Hybridization Analysis  (Genetic Engineering News, Volume 23, Number 2 January 15, 2002)
• Blocking Strategies for Nylon Membranes Used in Enzyme-linked Immunosorbent Assays (IVD Technology Magazine, Originally published July, 1997)
• Membranes for Transfer and Immobilization Family Data Sheet